FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5247783
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT9150.016.7123789
CGGGATT8150.016.5554110
AAACGGG15650.015.2314777
CGTTGGA15300.015.2085221
AACGGGA14100.014.9919878
GAAACGG16100.014.8064566
GGAAACG17300.014.2252475
CACCGCG3101.8189894E-1213.78295630
CGATAAG1000.007356029513.5547791
CGGGAAT5450.012.79120510
ACCGCGC3202.910383E-1112.64948831
CGCGCCT1607.871653E-512.64948833
CGTTAAA2603.511377E-812.1645471
ACGCCCG2801.0469557E-711.24399134
CGCCCGG4600.011.2439935
GCCACCG10650.010.55763728
CCGCGCC3451.5663318E-810.42920932
GACGATT3155.0183553E-710.03443052
CATCGTA2952.3347384E-69.9554881
GCGTGAG12150.09.43948922
Produced by FastQC (version 0.11.5)