FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6016738
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCG3850.014.02475130
GCCACCG9100.011.86699828
CGTTGGA16200.011.6050861
ACGGGAT10050.010.5946819
CGGGATT9400.010.37005610
GCGTGAG10500.010.14104622
ACAGGCG14300.010.06796418
CCCGTAA1808.9734E-610.0267951
CCACCGC7350.09.99912729
AAACGGG18900.09.918457
GAAACGG18900.09.7642996
CGTGAGC10700.09.67116923
GGAAACG18500.09.65117555
AACGGGT3750.09.5978858
CCCAACG1757.21791E-59.45383551
AACGGGA15150.09.403878
CCCAAGT18900.09.3105951
ATAGGCG1952.3967477E-59.22896718
AGGCGTG13750.09.16199920
CCCAAAG48650.09.1508671
Produced by FastQC (version 0.11.5)