FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6942081
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18950.013.4779261
AACGGGA17000.013.35940558
ACGGGAT13400.012.9211349
GAAACGG20450.012.6503866
CGGGATT11900.012.47129810
AAACGGG19900.012.4295187
GGAAACG21400.011.5055885
CGACACT2056.119756E-511.0255431
CCCCGGA2952.320572E-69.9603631
CGGGAAT7450.09.96029910
CCCGGAT2057.477036E-49.9229891
CCCAACG2301.9620289E-49.8271151
CGAGAGG4509.713403E-109.543221
CCCCGCG2000.00679260039.0409451
CCCGAAT6850.08.908961
ACCACGC12950.08.85554131
CCACGAT3857.9471283E-78.8061151
CGGGATA3104.7721172E-58.70430810
CGTATGC2606.6982576E-48.6932161
CCCAAGT32900.08.6562241
Produced by FastQC (version 0.11.5)