FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4488831
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA13700.012.5412571
ACGGGAT8400.011.7779279
CTCGTAT1650.001442020110.90188342
CGGGATT8100.010.82617710
AACGGGA11400.010.6508548
CGGGATA2151.0460833E-410.45820210
CCCGACT2201.2497124E-410.2760311
AAACGGG14000.010.1183117
GAAACGG13800.010.1052826
TCTCGTA1800.00297102849.99417241
TCGTATG2251.656402E-49.99339343
GGAAACG15000.09.9422985
CGTATGC1850.00372543679.72460244
CAACGAT1900.00464764569.46753131
ATAGGCG1900.00464807269.46742518
CCCCGGA2504.5191107E-49.0429071
CGTGAGC10950.09.03522423
CGATATT2300.002134148.8463231
ACGCCCG3104.773417E-58.70392334
CGCGCCC2851.8318884E-48.6785733
Produced by FastQC (version 0.11.5)