FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8195721
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20850.012.3824511
ACGGGAT14150.011.5543159
GATCGGA1703.73906E-711.4783291
AACGGGA19100.011.3084548
AAACGGG23500.010.7228517
CGGGATT12950.010.6558210
ACAGGCG17300.010.57748918
GCGTGAG12650.010.31567222
GGAAACG23850.010.1271165
GAAACGG23850.010.1252636
CGTGAGC13350.010.11183723
CGGGATA3051.364242E-109.83555710
GAGCCAC36150.09.54307626
TCGGAAG2701.832268E-89.4456853
ATAGGCG1757.437265E-59.42808418
ATCGGAA2403.2052412E-79.37708952
GCCACCG12700.09.33022728
CCCAACG2301.8022783E-69.1365971
CCCAAAG59100.09.0924261
AGGCGTA1500.00182604778.99953620
Produced by FastQC (version 0.11.5)