FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7695602
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT15500.012.1889049
CGTTGGA22050.011.562431
CGGGATT14650.011.36085910
AACGGGA20200.010.9116848
AAACGGG23900.010.7281297
GAAACGG24600.010.4241446
GGAAACG25000.010.2839555
CACCGCG5301.8189894E-129.76112930
GCGTGAG17500.09.51066222
CCGGATT2658.0291655E-59.3622222
CGTGAGC18150.09.29416123
ACCGCGC4702.3264874E-99.09298931
CCCAAAG85650.09.0353731
ACAGGCG26150.08.94496618
CCCGGTC2300.00217363758.8286421
AGGCGTG23500.08.80515620
GTCCGGC2300.00223577168.8012836
ACTGCGC6157.2759576E-128.77782831
GCCACCG15150.08.75983528
CCCGGTT3206.653526E-58.4607831
Produced by FastQC (version 0.11.5)