FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7744628
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5550.028.3773344
TCTCGTA6050.026.4039843
TCGTATG6200.025.40228745
TATCTCG6250.025.19906841
ATCTCGT7550.022.35016642
GTCACGT8250.019.6358929
GATCGGA8300.018.4512771
AGTCACG9500.017.99956728
ACGTGGC9300.017.66063732
ACACGTC9350.017.56619513
CGTCTGA9650.016.32063916
CGTGGCC9700.016.00456233
ATCGGAA9900.015.6829592
TCGGAAG10350.015.4359063
ACGTCTG10950.015.20491615
TCACGTG11650.014.29131630
CACGTCT12100.013.2019914
CGGAAGA11700.013.0778744
GCACACG13000.012.80730111
TACGCCC1300.003025031212.11493733
Produced by FastQC (version 0.11.5)