FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6759250
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22850.013.6227341
AAACGGG23050.013.5656637
AACGGGA19700.013.2463248
GAAACGG24100.012.7877556
GGAAACG24300.012.5899335
ACGGGAT14600.012.3273499
CGGGATT12650.012.27185910
CGGGAAT8950.010.80929210
AACGGGT4803.6379788E-1210.3106278
CGTATGA2453.3928896E-510.12741
CTCGACA2952.457713E-69.91572832
CGGGATA3206.532864E-79.84305410
TCTCGAC2453.871006E-49.18411131
ACGGGTT4203.3240212E-89.1060959
ACGCCCG4752.7994247E-98.99976434
CTCGACT2250.00182689218.9990312
CCGGCTA8400.08.83872638
GTTGGAA40650.08.7997912
TGTCGAA2606.835051E-48.67557051
GCGTGAG16150.08.63811922
Produced by FastQC (version 0.11.5)