FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071745

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071745
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6980995
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC170270.2439050593790713TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT20200.037.4234242
TCTCGTA20450.036.9664541
CGTATGC21050.036.01914244
TCGTATG22400.034.04922543
ATCTCGT22600.033.54928240
GTATGCC25450.029.8794145
AATCTCG26050.029.02014239
GATCGGA31750.024.144531
ACACGTC32650.023.49710313
CGTCTGA32700.023.2547716
TCGGAAG33200.023.1118143
ATCGGAA33450.022.9390832
ACGTCTG34600.022.3028915
CGGAAGA34900.022.0495534
CACGTCT35800.021.49246414
GTAATCT36300.020.95046237
GCACACG37350.020.7220511
CCTTGTA37600.020.40563433
CACACGT38200.020.26095612
AGTCACC39950.019.37287728
Produced by FastQC (version 0.11.5)