FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8178287
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG3350.022.83547635
CGTCGGA500.002257865922.5135631
GTCCCGA4000.019.12482836
TCTCGTA4950.017.27225543
CTCGTAT5050.016.484744
TCCCGAT4950.015.45440637
TCGTATG5600.015.26734645
CCCGATC5050.015.14810238
ATCTCGT5650.014.734200542
CCGATCT5400.014.16628139
ACCCGTC5950.013.6132432
ACGGGAT13950.012.4186359
CGTTGGA21850.012.36442851
ACCGCGC5500.012.27246731
AAACGGG22000.012.2720927
CCGCGCC5650.011.94672232
AACGGGA19250.011.9213888
GAAACGG23550.011.8478256
CCCGTCC7400.011.5538833
CGCGCCC4300.011.51145533
Produced by FastQC (version 0.11.5)