FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8644595
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGAT2206.148184E-1011.6079841
CGTTGGA23900.011.4394211
ACGGGAT14800.011.0453989
AACGGGA19200.010.7793958
AAACGGG24850.010.5623587
CGGGATT15000.010.49819510
CGTGAGC16600.010.48067123
GCGTGAG15950.010.43707722
CACCGCG3800.010.26240230
ACAGGCG21900.010.06676218
GGAAACG25400.09.9255245
GAAACGG26600.09.7026866
CCCAAAG66050.09.4158181
CGTTAGG1300.00565757659.2443681
GCTAGAC12650.09.01119429
CCCAAGT28350.08.954971
GCCACCG13150.08.89627128
AGGCGTG21050.08.69209120
GAGCCAC43800.08.5948626
CGTATGA1757.595926E-48.5840561
Produced by FastQC (version 0.11.5)