FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6864546
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22350.012.9165981
CGCGCCC4850.012.52569633
ACGGGAT14500.012.2559569
ACCGCGC6250.011.88105231
AAACGGG23900.011.4844487
AACGGGA20000.011.4725058
CCGCGCC6800.010.9191332
GAAACGG23950.010.898436
CGGGATT14050.010.88874110
CACCGCG7050.010.85186730
GGAAACG24150.010.7155
CGGGAAT7750.010.45071110
CCCGTAA2657.208966E-610.2129471
GCCACCG17100.09.341639528
AACGGGT4102.2500899E-89.3272418
CGCCCGG8000.09.28119435
CCCCGAT2453.7817244E-49.2055481
ACGCCCG4651.9081199E-99.19349134
CCCCGAA3155.557831E-58.5918451
CCCGGGT13250.08.5107891
Produced by FastQC (version 0.11.5)