FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12245804
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA35500.014.395521
CGTACGT3000.014.25171434
CCGTACG2905.456968E-1213.96793733
AAACGGG37250.012.5618817
ACGGGAT22400.012.5551599
AACGGGA30400.012.0635258
GAAACGG40250.011.6814846
CGCGCCC7200.011.56450333
CGGGATT21000.011.14228410
CCCGCGT1650.00142759510.9153051
GGAAACG41900.010.8991045
GATCGGA4152.401066E-1010.3070741
ACCGTAC4803.274181E-119.84545632
ACCGCGC9600.09.6110431
CGGGATA6100.09.22083810
CGCCCGG13450.08.86752635
ACAGGCG41700.08.73938118
ACCGTGC11750.08.61851531
CACCGCG10200.08.604115530
AACGGGT8900.08.5950648