FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6920380
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA22200.014.2472251
AAACGGG23850.013.106917
ACCGCGC5300.012.73322831
AACGGGA19650.012.7038218
CGGGATT13700.012.63991210
CCGCGCC5350.012.61422632
CACCGCG5200.012.54322930
ACGGGAT14000.012.5296949
GAAACGG24650.012.3170426
ATAGGCG2251.0475451E-611.99423818
GGAAACG24750.011.9398625
CGCGCCC3855.456968E-1211.68599133
ACGGGTT3652.8558134E-1011.0905629
CCCGAAA2301.660506E-510.8048831
GCGTGAG15650.010.63394522
GCGTAAG1700.001846712910.58322722
AACGGGT4509.094947E-1210.4949588
GGCGTAA1500.0088158910.49495721
CGCGCCT2151.04138264E-410.46303833
CGTGAGC16750.010.06986223
Produced by FastQC (version 0.11.5)