FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8064917
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGG25850.011.4887667
GAAACGG27450.011.2288576
CGTTGGA23300.011.2059311
CGGGATT14300.011.1706810
ACGGGAT15700.011.0344619
AACGGGA21550.010.9623098
GGAAACG28550.010.3258285
GATCGCG2902.162386E-59.30986336
AACGGGT5801.8189894E-129.3098638
ACCCGGC7650.09.11717536
GCCACCG18500.08.87764328
CCCGGAT3155.6901303E-58.5746531
ATCGCGC2902.1978289E-48.53404137
GCGTGAG19550.08.17067922
CACCCGG8300.08.13210935
ACGGGTT5352.3492248E-87.990249
CCACGAA4551.0785752E-67.91506431
CGTGAGC20800.07.89598123
CCCAAAG90900.07.7256771
TACAGGC71600.07.6989117
Produced by FastQC (version 0.11.5)