FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6156852
Sequences flagged as poor quality0
Sequence length36
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2550.018.8644981
AGTCACG3100.015.96441828
ATCGGAA3350.014.7820482
TCGGAAG3400.014.5646643
GTCACGT3300.014.087975529
ACACGTC3650.013.14794713
CGTTGGA15350.012.5353671
ACGTCTG4250.012.35036915
AACGGGA12500.012.1174768
CGTCTGA4050.011.84938416
AAACGGG15500.011.6104867
CACGTCT4150.011.56385714
GCGTGAG10300.011.50255322
GAAACGG16250.011.2607496
GCACACG4650.010.96545911
ACGGGAT9350.010.906829
CGTGAGC11450.010.6092323
CGGAAGA4850.010.5195114
GGACCGC1000.00879793710.49781525
TCACGTG4450.010.46051630
Produced by FastQC (version 0.11.5)