FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5839355
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT3550.018.37255542
TCTCGTA3750.017.39268541
CGCCAAT3850.016.94324933
TATCTCG3850.016.94107439
CGTATGC4150.015.71628444
GTCACGC4450.014.65675829
ACGCCAA4650.014.51201632
TCGTATG5000.013.49432643
GATCGGA4350.013.4917211
ATCTCGT4900.013.310842540
AGTCACG5750.012.90761628
GAAACGG17400.011.8952196
CGTTGGA16650.011.7947381
ATCGGAA5450.011.5897062
CGTCTGA6100.011.4296216
ACACGTC6600.011.24527213
GGAAACG18150.011.186055
ACGGGAT11650.011.0039579
AACGGGA14100.010.8465028
AAACGGG18250.010.8447547
Produced by FastQC (version 0.11.5)