FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11111218
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG5950.024.5723633
GATCGGA7450.019.990431
CTCGTAT7350.019.89047844
TCTCGTA8100.018.88178643
TCGGAAT7850.018.33787737
GTTTCGG8400.017.6742334
TCGTATG8600.017.03514145
ATCTCGT8950.016.33463942
ATCGGAA9550.016.2945022
TTCGGAA9650.016.08413336
ACACGTC10500.015.42256213
TTTCGGA9750.014.99631635
CGTCTGA10350.014.34223716
GTCACGT10650.014.14941729
TCGGAAG10900.014.069473
AGTCACG11450.013.75010128
ACGTTTC12800.012.8281332
CGTTGGA29100.012.7945621
CGGAATC12050.012.13291238
TCACGTT13400.012.08587630
Produced by FastQC (version 0.11.5)