FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11675314
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5300.020.37683345
CCGATGT6800.016.54510932
CGATGTA7000.016.0723233
CTCGTAT7150.016.04915641
TCTCGTA7100.015.844933540
ACCGATG7200.015.626739531
TATCTCG7200.015.62566838
CACCGAT7700.014.31995930
GTCACCG8200.014.26926928
CGTATGC7750.014.22574443
CGTTGGA29450.012.9912861
ATCTCGT9050.012.680099539
TCGTATG9400.012.44696842
ACGGGAT19350.011.8603129
TCACCGA9750.011.77069329
AAACGGG31850.011.6556017
AACGGGA25700.011.6443178
GAAACGG34900.010.7014556
GGAAACG35000.010.4148215
ACACGTC11050.010.38430212
Produced by FastQC (version 0.11.5)