FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7804224
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT12350.032.7930744
TCTCGTA12350.032.61088643
TATCTCG13200.031.19255341
ATCTCGT13450.030.27839542
TCGTATG13600.029.77945
GATCGGA13550.029.4080731
ATCGGAA15300.026.4742952
ACACGTC15850.026.1190113
CGTCTGA15950.025.95525616
TCGGAAG15300.025.7388973
ACGTCTG17750.023.95695515
CACGTCT18250.023.42388214
CGGAAGA17150.023.2248234
GCACACG20450.020.3537211
CACACGT21150.019.68007512
CCGGATC1352.6518834E-413.33522
AGAGCAC32350.013.2143168
TCTGAAC34700.012.83819318
AGCACAC33550.012.74167310
GTCACAT33650.012.57016429
Produced by FastQC (version 0.11.5)