FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6921389
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA15900.011.6281921
CGTGAGC12450.011.20406323
GCGTGAG12400.011.12820222
CCCGAAT3700.010.5627211
CCCAAAG58500.010.3550791
CCCAAGT22300.010.0435121
GAGCCAC33300.09.99932526
ACGGGAT10400.09.95117959
ACAGGCG16150.09.93733918
AACGGGA13100.09.7320998
CGTTATA1705.2885403E-59.7263071
CGGGATT9650.09.63658610
ATAGGCG2209.944906E-79.54474318
CCCTGAA19850.09.5414471
CCCTAAA17350.09.5300991
AGGCGTG15500.09.48316320
GGCGTGA15500.09.192862521
AAACGGG17800.09.1847087
GAAACGG18700.08.9030786
CCCTATT11000.08.8822881
Produced by FastQC (version 0.11.5)