FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10503141
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA4250.011.0499271
CGTTGGA23350.010.7698051
AAGCGGC5900.010.1660323
AACGGGA18350.09.8869848
AAACGGG23850.09.8073297
ATCGGAA5250.09.4253642
ACGGGAT14350.09.4038389
CCCCGTA1450.00122440269.4028851
GAAACGG24250.09.3985956
ACACGTC5200.09.22701413
CAAAGCG13750.09.0343681
GGAAACG24450.09.0153375
CGGCTGA7700.08.76300056
ACAGGCG18850.08.51116518
AGCGGCT7050.08.5077434
AAAGCGG8000.08.43462852
CGTCATT5600.08.3861061
CCCAAAG73950.08.317151
CGGGATT13250.08.26078810
TCGGAAG6050.08.1790343
Produced by FastQC (version 0.11.5)