FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5902161
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA17200.013.131441
AAACGGG18150.012.6389167
AACGGGA14100.012.6006878
CGTCCCG1454.8597986E-412.4119735
ACGGGAT10150.012.4081849
CGCCAAT1300.00293877212.1617341
GAAACGG18700.012.1474296
GGAAACG18250.012.1112065
AACGGGT4051.8189894E-1211.6613968
ACCGCGC5600.011.64997731
GTCCCGA1558.5119414E-411.61119836
CGCGCCC4600.011.24834633
CGGGATT10050.010.96519310
CGAAACG1650.00140578110.9352885
CGCCCGG7450.010.87088635
ACAGGCG21950.010.86069418
GCGTGAG16050.010.78988122
CGGCCTA2108.214098E-510.71071539
ACGCCCG2952.1408778E-710.67639734
CACCGCG5400.010.412930530
Produced by FastQC (version 0.11.5)