FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7053357
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC3750.018.39863829
AGTCACG4550.015.16371328
ACACGTC4750.014.52513913
GATCGGA4700.014.394621
CGTCTGA4850.014.22565116
ATCGGAA5250.013.1417922
ACGTCTG5400.012.49898715
TCGGAAG5750.011.9990283
CGTGAGC10500.011.85626623
CGTTGGA17750.011.6040011
ACGGGAT10550.011.37348659
CACGTCT6150.011.21860314
AAACGGG19200.011.0928517
GCGTGAG10850.011.05901122
AACGGGA15000.010.9991088
GGAAACG18650.010.9374515
GAAACGG19400.010.9011786
CCACGAT3101.8189894E-1210.669561
CGGAAGA6750.010.4435984
GCCACCG10600.010.18792628
Produced by FastQC (version 0.11.5)