FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5727673
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA16700.013.6383221
AAACGGG17750.012.5482487
CGTGAGC12500.012.41831323
GCGTGAG12450.012.28748822
AACGGGA14050.012.0084168
AACGGGT4051.8189894E-1211.6644718
CGTAGAT2158.018365E-611.5374611
ACAGGCG17750.011.53304118
GAAACGG19450.011.4616956
ACGGGAT11400.011.4452149
TCTCGTA3602.237357E-1011.24798243
CGGGATT10400.011.24788310
GGAAACG19450.010.9999415
CGCCCGG5550.010.53855735
AGGCGTG16100.010.47939420
CGGGTTT4152.419256E-1010.29926610
CACCGCG4406.548362E-1110.22534830
GCCACCG10150.010.19521428
ACGCCCG2901.980694E-610.0843134
CGTATGC3909.938958E-99.80593245
Produced by FastQC (version 0.11.5)