FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8097816
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21950.012.7446751
CGGGATT15350.012.60240210
ACGGGAT16150.012.117499
TATGCCG2351.7373495E-611.50140545
AACGGGA20600.010.9193998
AAACGGG24250.010.8528247
CGTATGC4253.274181E-1110.58513244
TCTCGTA4101.9463187E-1010.423902541
GAAACGG25350.010.29315956
CTCGTAT4203.259629E-99.64003142
GGAAACG26900.09.4671995
GTCCGGC2150.00120882679.416018536
CCGGCTA7900.09.11142238
CGGCTAA8650.08.32141439
ACAGGCG26000.08.21920118
ATCTCGT5201.4228135E-88.21884540
GATCGGA5801.3169483E-98.1683231
GCCACCG16700.08.08166328
TCGTATG5301.9970685E-88.06367343
CGCCCGG7850.08.02327435
Produced by FastQC (version 0.11.5)