FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8266811
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT8150.028.70961242
GATCGGA8050.027.9704821
CGTATGC8450.027.1578344
TCTCGTA8750.026.22670441
ATCGGAA8850.025.9466762
TCGTATG9450.024.76014143
TCGGAAG9300.024.6905943
ATCTCGT9450.024.04590640
ACACGTC9650.023.7806913
CGTCTGA10250.021.94965616
ACGTCTG11150.020.58149315
CGGAAGA11500.020.3573364
CACGTCT12250.018.549714
AATCTCG12900.018.31264939
GCACACG13500.017.33209811
GTATGCC13800.017.28221345
CACACGT14150.016.85392212
CGTTGGA23700.013.3007311
ACGGGAT16450.012.0356179
AACGGGA21950.011.9923128
Produced by FastQC (version 0.11.5)