FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10020893
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA8900.024.6174471
CTCGTAT10150.020.1638442
CGCCAAT10150.020.16212833
TCTCGTA10450.019.8001941
TATCTCG11200.018.875939
CGTATGC11250.018.39098444
ACGCCAA11300.017.91139432
GTCACGC11450.017.67674629
TCGTATG12050.017.35776343
TATGCCG5950.017.05860345
ATCGGAA12850.017.0422732
TCGGAAG13000.016.1509673
ATCTCGT13000.016.0893140
CGTCTGA12700.015.93627416
AGTCACG13400.015.60778628
CGTTGGA28800.015.0580951
CGTCCGA1502.5913432E-614.9927256
ACACGTC14350.014.41737113
AAACGGG30850.014.0685767
AACGGGA26750.013.9550788
Produced by FastQC (version 0.11.5)