FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7738889
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATG119820.1548284256306041TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT14450.035.18698544
TCTCGTA14650.034.7066243
TCGTATG15450.032.90908445
CGTTTCG15700.032.3888233
ATCTCGT16250.031.56624842
GTTTCGG16400.030.86917934
TCGGAAT16750.030.49280737
TTCGGAA16850.029.91124536
TTTCGGA17950.028.20359235
AATCTCG18750.026.99849741
GTCACGT19200.026.71743429
AGTCACG20050.025.58378228
ACGTTTC21050.024.58422332
CGGAATC20750.024.28811638
GATCGGA22050.023.9328171
TCACGTT22050.023.77449430
ACACGTC22400.023.00035713
CGTCTGA22750.022.8442916
TCGGAAG23900.022.219783
ATCGGAA24150.021.9897632
Produced by FastQC (version 0.11.5)