FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6284885
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18000.014.4481291
AAACGGG19050.012.7530717
AACGGGA15900.012.7329188
ACGGGAT11000.012.6788999
GAAACGG20150.012.3985936
GGAAACG21400.011.779555
CGTTCGA1852.8869213E-411.0016311
CCGGCTA6750.010.9977338
CGGGATT10850.010.78092810
GGCGCGA2352.2076902E-510.52999319
CGGCTAA7550.010.42822839
CGGGAAT7750.010.15895210
ACCACGC10300.010.0485531
ACGCCCG3451.7354068E-79.78129134
AACGGGT3858.5163265E-89.3484978
GGCGCCC5557.2759576E-129.32262321
ACCCGGC6350.09.21062536
ACAGGCG22900.09.13581518
GATTTCG7650.09.1206275
CGCCCGG6301.8189894E-128.92665935
Produced by FastQC (version 0.11.5)