FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5041825
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA13650.014.8326548
ACGGGAT10750.014.6486849
CGTTGGA16150.014.4006851
AAACGGG17400.013.5752887
CTCGTAT2501.4879333E-913.49918841
CGGGATT10250.013.3881110
TATCTCG2857.748895E-1012.63257338
GAAACGG18500.012.5250316
TCTCGTA2951.3387762E-912.20180840
GGAAACG19250.012.1539075
ATAGGCG2054.8435413E-612.07124718
CGCCAAT2452.2447966E-711.93864932
TATGCCG2604.849571E-711.24965745
GCGTGAG13200.010.73767122
CGCCCGG6650.010.48954735
CGAAGAT4406.002665E-1110.2635071
ACGCCAA2851.5842761E-610.26152231
TAGGCGT2006.222558E-410.12318619
CCCCGGT1800.002871418110.0354291
ACGCCCG3451.7301863E-79.78318534
Produced by FastQC (version 0.11.5)