FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3895890
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCCG1709.491032E-613.22518134
CCGGCTA3850.012.84847338
CGTTGGA11050.012.0887971
GAAACGG11050.012.0054746
AACGGGA9950.011.0719368
CGCCCGG3703.6379788E-1010.93856535
AAACGGG12250.010.8284557
GGAAACG12350.010.2615375
ACGGGAT7500.010.1922069
ACCACGC7100.09.49977831
CCGCGCC2851.7746948E-59.46644632
CGTGGAT1950.00543865569.2885681
CGCCACC5007.4942363E-108.99312427
CGGGATT7300.08.93152710
CGGCTAA4304.909998E-88.88935139
CCCGAAA2050.0082006848.8354661
CCACGCC7950.08.76688132
CGAAGAA3104.4015578E-58.7642131
CGCCTGG5659.094947E-118.75515535
CCCGGCT5659.094947E-118.75515537
Produced by FastQC (version 0.11.5)