FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4973471
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1751.8075769E-411.57087544
AACGGGA12850.011.3800928
CGTTGGA16450.011.3691871
ACGGGAT9250.011.1883179
AAACGGG15750.010.5702857
GGAAACG17000.010.4567495
GAAACGG16500.010.3642866
CGATGAA2453.4316603E-510.1168151
CGAGAAT2952.4132096E-69.9297551
GATTTCG7050.09.5752625
CGGGATT8550.09.47294510
CCCCGAA2453.816154E-49.1971041
ACCGCGC3451.8196752E-69.12990631
CGTAGAA2250.00180082039.0131621
CACCGCG3901.0211897E-68.65386730
CGCCCGG4803.1866875E-88.43718135
GCCACCG10950.08.42459428
GAGCCAC39100.08.40155526
CGGGATA2700.00102001848.33268410
ATTTCGT8400.08.3040926
Produced by FastQC (version 0.11.5)