FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9582149
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT14350.014.5812989
AACGGGA20350.013.9306458
CGGGATT14100.013.88227710
CGTTGGA25600.013.4757691
AAACGGG26950.012.6896577
GAAACGG28250.012.5835016
CCCCGAT3356.002665E-1112.1151771
GGAAACG29750.010.965895
GCGCGAT4203.246896E-99.64211820
GGCGCGA4355.9371814E-99.30972819
CGCGATC4651.9099389E-99.19284421
GATTTCG10350.09.1300215
AACGGGT6851.8189894E-128.53980358
ACAGGCG32000.08.36663318
CCGGATT3309.818877E-58.1833692
CTACGTC3309.84817E-58.18127635
TGGCGCG5606.213668E-98.03518218
CCTCGTA2550.0055520967.95800831
CGGGAAT9400.07.898536710
CCCGATG2600.0067238467.78993752
Produced by FastQC (version 0.11.5)