FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071880

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071880
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8672131
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10150.030.37101445
TCTCGTA10400.029.63974642
CTCGTAT10150.029.26140243
GTCACGA10700.028.5966328
TATCTCG11000.027.81944740
ACGAGTG12350.025.14095931
AGTCACG12600.024.99984227
CACGAGT12700.024.62440730
ATCTCGT12700.024.09447541
TCGTATG12350.024.04870444
ATCGGAA12900.023.9029141
ACACGTC13050.023.61863112
CGTCTGA13350.023.4247915
TCGGAAG13650.022.5898282
CGGAAGA14300.021.7195343
ACGTCTG14750.020.89636414
CACGTCT16050.019.20393413
GCACACG17000.018.92536710
CGAGTGG17450.018.05083732
CACACGT19500.016.49903911
Produced by FastQC (version 0.11.5)