FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7398979
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21850.013.7227481
ACGGGAT13950.013.7093219
AAACGGG23550.012.993037
AACGGGA19800.012.8405758
GAAACGG24350.012.2894586
CGGGATA2851.0462827E-811.84179810
CGGGATT13500.011.66636410
GGAAACG25900.011.3802435
CCCGCAA2952.0794687E-710.699091
TCGCATC1800.00295773669.9997410
CGGGAAT8800.09.97133210
ACTGCGC6200.09.4349231
CCCGAAA3155.5327855E-69.3040841
CCGAGTA12350.09.1098142
CGCTAAT2250.00179261559.0178051
GCCACCG16450.08.89004628
CCCGAGT17800.08.7391651
AACGGGT4803.187415E-88.4372818
ACCACGC12900.08.371592531
ACAGGCG22300.08.0714318
Produced by FastQC (version 0.11.5)