FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6071485
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCCC3750.013.19260533
CGTTGGA16600.013.0790871
ACCGCGC4950.012.26494331
CGCCCGG6100.011.428020535
CACCGCG4950.011.35642930
CCGCGCC5750.010.55860232
CGTCCAA1500.00845597610.5540971
CACCGAT1750.00224293210.3387071
AAACGGG17750.010.2607557
AACGGGA14400.010.1492228
GAAACGG18750.010.0761786
GGAAACG19250.09.97641855
ACGGGAT11100.09.9255599
CGGGATT10950.09.44551510
AACGGGT3603.2473872E-79.3685138
GCCACCG13500.09.32787328
ACGCCCG3451.8324099E-69.125319534
ACAGGCG22300.09.07443418
GCGTGAG16900.09.04795822
CGTGAGC17150.09.04718323
Produced by FastQC (version 0.11.5)