FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6508763
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG580180.8913828941075286TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA66950.043.0265271
AACGATC68350.042.4516238
TCTCGTA68600.042.36118743
CTCGTAT68750.042.23604644
TCGTATG69000.042.2286645
GTCACGT69600.041.88181329
ATCTCGT69300.041.83592642
AGTCACG69800.041.72958428
TCGGAAG69250.041.707223
ATCGGAA69200.041.7047732
CGGAAGA70850.041.401814
ACACGTC71450.041.36402513
CGTCTGA71500.041.20927416
AAACGAT71000.040.9305137
ACGTGAA71900.040.79796232
ACGATCT71100.040.7147739
CGTGAAA71900.040.704133
ACGTCTG72600.040.6158715
TCACGTG71900.040.45380830
CACGTGA72350.040.38874431