FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4535165
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT5000.027.43451144
TATCTCG5400.025.40456841
GTCACGA5800.024.03788829
TCGTATG6100.022.92824645
TCTCGTA6100.022.48928643
GATCGGA6500.021.2054041
ATCTCGT6750.020.32365442
CACGAGT7250.019.23052431
ACACGTC7250.019.22988713
ACGAGTG7550.018.7642432
ATCGGAA7350.018.744152
TCGGAAG7500.018.0683333
AGTCACG7900.017.36342428
CGTCTGA8000.016.8649216
ACGTCTG8250.016.62642715
GCACACG8900.015.91745311
CGGAAGA8900.015.733664
TCACGAG9400.015.31050630
CACGTCT9300.015.23283114
GGATATC10300.013.75543338
Produced by FastQC (version 0.11.5)