FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071910

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071910
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6474048
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGGA15150.012.7660878
CGTTGGA18450.012.6018961
AAACGGG19350.012.4358317
ACGCCCG3304.9112714E-1112.267158534
ACGGGAT11850.011.9562289
GAAACGG20250.011.6653376
CGGGATT11500.010.75565410
GGAAACG21000.010.74344355
CCGGCTA6500.010.72556838
CGGCTAA6550.010.64377539
CCCGGAA4051.6352715E-910.03257751
CGCCCGG6300.09.99546235
TATCTCG3206.5676977E-79.83920841
CTCGTAT2851.768925E-59.46931344
CCCCGAT2150.00117041329.4492881
CGTGAGC17300.09.35969523
AACGGGT4102.2599124E-89.3247478
ACGGGTT4152.7526767E-89.21249
ACAGGCG22450.08.91549918
CCCGAAT7150.08.8398931
Produced by FastQC (version 0.11.5)