FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071915

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071915
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6391442
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21600.012.633891
AAACGGG22800.011.9408517
AACGGGT4150.011.9258148
ACGGGAT14000.011.7302769
CCACGAT3102.8121576E-911.6402881
GAAACGG23100.011.105176
AACGGGA19500.010.9597948
CGGGATT13000.010.73032110
GGAAACG24150.010.4360625
ACGGGTT4101.9463187E-1010.4251719
CGATGAA3155.094025E-710.02358151
CCACGTA2851.7109207E-59.4960251
CCGGCTA7150.09.43905838
CGGCTAA7450.09.36202539
CCGGTTT2751.2321226E-49.0015273
ATGCCCG5302.3646862E-108.91500234
ACGCCCG3301.0229913E-58.863543534
CGCCCGG6351.8189894E-128.85817535
CGAAGAT7000.08.6990381
ACAGGCG23500.08.61511218
Produced by FastQC (version 0.11.5)