FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5332659
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3550.022.9189991
CGTATGC2700.019.21313745
TCTCGTA4600.017.59974143
CTCGTAT4700.017.2252844
CCCTACG1451.7294296E-615.5866751
ACACGTC5100.015.43057413
ATCTCGT5300.015.275104542
TCGTATG5300.014.89451545
TATCTCG5300.014.42621841
GTCACGA5500.013.89954229
AGTCACG5850.013.45229628
TCGGAAG6600.012.6658993
CGTCTGA6400.012.64756116
CGTTGGA13950.012.6369391
ACGAGTG6100.012.53484232
CACGAGT6450.012.20332231
CGGAAGA7250.012.1535984
ATCGGAA6750.012.049722
ACGGGAT9750.011.7613719
AAACGGG15350.011.572837
Produced by FastQC (version 0.11.5)