FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071930

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071930
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5737469
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA17100.014.8065181
ACGGGAT9950.014.2369989
AAACGGG16700.013.8682517
AACGGGA14300.013.3654578
CGGGATT9700.012.98127210
GAAACGG17850.012.6022536
GGAAACG19350.011.9102795
ACGCCCG4500.010.99296634
TATCGCA1650.001441975210.902029
CGGGATA2301.74958E-510.75389510
AACGGGT3401.2501914E-810.5813728
GCGTGAG13600.09.75487322
CCCGTAA2555.138656E-59.7517711
CCGGCTA6700.09.73308738
GATTTCG6550.09.6587185
CGGCTAA7000.09.63719539
ACCACGC11900.09.44781831
CGCCCGG7200.09.36941335
ACAGGCG20550.09.0817818
CGTGAGC15500.08.84925423
Produced by FastQC (version 0.11.5)