FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5162262
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA15850.015.0504871
CGGGATT10100.014.03407410
ACGGGAT11250.013.999429
AAACGGG17900.013.3243757
GAAACGG18850.013.2504576
AACGGGA14750.013.1181018
GGAAACG19650.012.1384325
CGCGCCC2901.6882586E-710.86183133
CGAGGAT2453.4792884E-510.1041681
AACGGGT4102.1391315E-99.8776418
CGTGAAT2555.398632E-59.7079261
CGTTGGT2109.7027567E-49.6448881
CCCGAAA2352.5454306E-49.576022
CCTCGAA3309.904234E-79.5474641
GCCACCG10200.09.48490228
CCCGAAT4601.5625119E-99.2954361
AAGCGGC4601.5661499E-99.2945353
ACCGCGC3951.2590135E-79.11363531
CTCGTAT2000.0070473159.00041343
GTTGGAA30800.08.9137822
Produced by FastQC (version 0.11.5)