FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071935

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071935
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4005076
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG1454.783942E-918.6202935
CCCGATC1855.631591E-915.81126338
CTCGTAT2052.1793312E-814.26994844
TCCCGAT1951.8320497E-713.8465537
CGTTGGA12050.013.4468861
ACGGGAT8300.013.0114469
TCTCGTA2257.391645E-813.00085743
AAACGGG12500.012.95947
AACGGGA11350.012.8848668
CGGGATT8000.012.09318910
GAAACGG13250.012.0563466
CCGATCT2251.0398217E-612.00124239
GTCCCGA2301.3568333E-611.73946736
GGAAACG14400.010.781025
CACCCGT2759.993582E-710.63613731
ACGGGAA4457.2759576E-1210.6174869
ATCTCGT2801.2576293E-610.4476442
CGGGAAT3909.0039975E-1010.38413410
CCCATAA11500.09.3933321
CTACGGT1950.00571522449.2327621
Produced by FastQC (version 0.11.5)