FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465487
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGC36570.10552629399562025TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC5600.034.5933545
GATCGGA5450.030.2194731
CTCGTAT9050.021.37494343
TCTCGTA9200.021.2700142
TATCTCG9100.021.25596240
TCGTATG9200.021.02643844
ATCTCGT9600.020.14946441
GTCACGA8950.020.10641128
ACACGTC9900.019.08282912
ACGTCTG10250.018.8700614
CGTCTGA10300.018.56010415
CACGTCT10450.018.2936913
ACGAGTG9500.018.2296531
ATCGGAA10450.018.1352481
TCGGAAG10500.018.0491522
AGTCACG10100.018.03978227
GCACACG10850.017.82655310
CACGAGT10350.017.1671430
CGGAAGA11050.017.1507783
CACACGT12450.015.89688411
Produced by FastQC (version 0.11.5)