FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071958

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071958
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7527908
Sequences flagged as poor quality0
Sequence length36
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5950.024.2480561
GTCACGA6500.021.46024529
ATCGGAA7100.021.1253492
TCACGAT6850.020.5828430
TCGGAAG7200.019.9986633
ACACGTC7150.019.92873813
CGGAAGA7700.019.0896324
CGTCTGA7400.018.85009216
AGTCACG8000.017.6239428
ACGTCTG8200.017.01105715
CACGTCT9050.015.74480314
CACACGT9350.015.40004112
GCACACG9200.015.32506311
GCGTGAG13450.013.49352222
ACAGGCG17150.013.46848818
CGTGAGC14350.012.85628323
ACGGGAT9700.011.906429
AGGCGTG16300.011.77835520
GGCGTGA16000.011.71796721
CGTTGGA15700.011.5826641
Produced by FastQC (version 0.11.5)