FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103223
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA8550.016.0889681
ACGGGAT4900.015.14991959
CGGGATT4550.014.33745610
AAACGGG9700.013.9141917
AACGGGA7350.013.772218
GAAACGG10050.013.2177186
CGCCCGG3303.6379788E-1212.95436435
ACGCCCG1751.269305E-512.85696334
GGAAACG9850.012.5719895
CCGGCTA3253.6379788E-1112.46136538
GCCCGGC7100.012.35898236
AACGGGT2452.2503991E-711.9359158
ACCGCAC2706.276787E-811.66631531
ACAGGCG11550.011.10105818
GCGTGAG8150.011.04065622
AGGCGTG10350.010.21477920
CGTGAGC8600.010.20137323
GCCACCG7100.010.1390728
CAGGCGT10250.010.0949819
CGTGTAA1800.002901398810.0226351
Produced by FastQC (version 0.11.5)