FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6978348
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC7600.028.41979433
ACGGCTA7650.027.9399432
CGTATGC8250.026.18084544
CTCGTAT8450.025.56099942
TCTCGTA8650.025.49020241
TCGTATG8900.024.52138743
GATCGGA9100.024.2526341
GTCACGG9450.022.85613329
ATCGGAA10200.021.4004042
ATCTCGT10400.021.20098340
AGTCACG10500.020.78479628
ACACGTC11200.020.69104813
CATCTCG11000.020.4536439
TCACGGC10250.020.4137330
TCGGAAG10900.020.0259253
CGTCTGA11350.020.02114116
ACGTCTG12550.018.28604315
CGGAAGA12300.017.7465524
GTATGCC12750.017.4699445
CACGTCT13050.017.41302314
Produced by FastQC (version 0.11.5)