FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006071967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006071967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6830858
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT11700.016.5303449
CGTTGGA21000.014.7383631
AAACGGG21250.014.6045947
AACGGGA17950.014.4079678
CGGGATT12900.013.77230710
GAAACGG23100.013.4367326
GGAAACG23250.012.6180665
CGCCCGG8050.011.73345535
AACGGGT4601.8189894E-1210.7555898
GCGTGAG17500.010.15245522
CGCGCCC5101.8189894E-1210.14237733
CCCGAAA2751.0978261E-59.8581751
CCCGTAA3257.630988E-79.7317881
CGAACTT2352.4477614E-49.6134691
CGTGAGC18250.09.48883923
GCCACCG16400.09.46230228
CCCCGAT2403.024124E-49.4131871
GTTGGAA39750.09.2537912
ACCGTGC6650.09.13098931
ACAGGCG24900.09.1219618
Produced by FastQC (version 0.11.5)